Synthesis of Host Ribosomal RNA's in Tabacco Leaves Inoculated with Tobacco Mosaic Virus

The synthesis of ribosomal ribonucleic acids (rRNA's) in tobacco mosaic virus (TMV)-infected tobacco leaves at different times after inoculation was analyzed using methylated albumin kiselguhr (MAK) column. At an early stage after inoculation, specific radioactivities (cpm/optical density at 260mμ) of 18s and 28s rRNA's increased in infected leaves. After 24hrs of infection, specific radioactivity of 18s rRNA from infected leaves was about a third of that of the same RNA from healthy tobacco leaves. Specific radioactivity of 28s rRNA from infected leaves was 70% of that of the same rRNA from healthy leaves at 24hrs after inoculation. Afterwards specific radioactivity of 28s rRNA from infected leaves was 80-100% of that of the same RNA from healthy leaves