Reproductive characteristics of the interspecific hybrid Sambucus javanica × S. ebulus

V magistrski nalogi smo primerjali glavne morfološke lastnosti listov in reproduktivnih organov starševskih vrst (S. javanica \u27ES\u27 in S. ebulus) ter njunega medvrstnega križanca (S. javanica × S. ebulus). Ker je križanec sterilen, je bil eden od pomembnih ciljev raziskati vzroke sterilnosti na nivoju materinskih celic pelodnih zrn. Ugotovili smo, da so nekatere morfološke lastnosti križanca precej podobne morfološkim lastnostim starševskih vrst. Citološka analiza, ki je temeljila na opazovanju kromosomov mikrosporocit, je pokazala, da sta bila oba starša (S. javanica ES in S. ebulus) diploida z 2n = 36, medtem ko smo pri medvrstnem križancu opazili velike variacije v številu kromosomov in nepravilnosti v celični delitvi. Prisotna je bila anevploidija, in sicer od hiper-diploidije do hipo- in hiper-tri- in tetraploidije. Na podlagi citološke analize sklepamo, da je sterilnost medvrstnega križanca posledica anevploidije, ki je povezana z nepravilnostmi v mikro- in makrosporogenezi.In the presented MSci thesis, we compared main morphological and reproductive characteristics of the parental species S. javanica \u27EC\u27 and S. ebulus, and their interspecific hybrid (S. javanica × S. ebulus). We also analyzed the reasons of reproductive sterility of the hybrid plants. It was found that some morphological characteristics of the hybrid plants were more similar to one or another parental species, whereas the others varied between the parental species. The cytological analysis based on microsporocytes showed that both parents (S. javanica and S. ebulus EC) were diploids with 2n = 36. The interspecific hybrid plants exhibited various types of aneuploidy (variations of the number of chromosomes such as hypo- and hipper- di-, three- and tetraploidy) as well as abnormalities in cell division. The investigation showed that sterility of interspecific hybrid plants was probably caused by aneuploidy and its association with irregularities in micro- and macrosporogenesis