VALIDATION OF THE GRAVIMETRIC METHOD FOR THE IDENTIFICATION OF HEAVY VOLATILE LIPOPHILIC SUBSTANCES IN THE WASTE WATERS

Namen diplomske naloge ja potrditev pravilnosti pri določanju težkohlapnih lipofilnih snovi v odpadnih vodah in izlužkih odpadkov, v koncentracijskem območju med 10 mg/L in 500 mg/L. Za določanje masti in olj lahko uporabljamo gravimetrijsko metodo, ki zajema skupino snovi, ki jih lahko ekstrahiramo z izbranim topilom pri definiranih pogojih dela. Ekstrakt zajame le nepolarno - lipofilno zmes snovi iz vzorca. Določanje težkohlapnih lipofilnih snovi in njihova kvantitativna določitev je osnovana na ekstrakciji z izbranim ekstrakcijskim sredstvom. Po ekstrakciji z odparevanjem topilo odstranimo in s tehtanjem preostanka določimo množino ekstrahiranih snovi, ki predstavljajo težkohlapne lipofilne snovi v vzorcu. V prvem delu diplomske naloge smo vpeljali metodo za laboratorijsko delo, nato pa bomo metodo ovrednotili oziroma izvedli postopek validacije, kjer bomo določili: mejo zaznavnosti, mejo določljivosti, pravilnost, ponovljivost in obnovljivost na različnih vzorcih odpadnih vod.The purpose of the diploma thesis is to confirm trueness in determining heavy volatile lipophilic substances in waste waters and extracts of waste in the concentration range between 10 mg/L and 500 mg/L. For determining grease and oil we can use the gravimetric method, which includes a group of substances that can be extracted using a particular solvent under defined conditions of work. The extract contains only the non-polar lipophilic mixture of the substances in the sample. Determining heavy volatile lipophilic substances and their quantitative determination is based on extraction using the selected extractant. After the extraction we remove the solvent through evaporation, and weigh the residue to determine the amount of extracted substances, which represent heavy volatile lipophilic substances in the sample. In the first part of the diploma paper we have introduced the method for laboratory work. Next, we are going to evaluate this methodin other words, carry out the process of validation. In the validation process we are going to determine the limit of detection, the limit of quantitation, trueness, repeatability and reproducibility of results of different samples of waste waters