Supercritical fluid adsorption and desorption of lipids on various adsorbents

In present work the feasibility of using supercritical fluid chromatography (SFC) for separation of free fatty acids (FFA) and triglycerides has been examined. Lipids such as oleic acid and sunflower oil (containing 96% of triglycerides) were usedadsorption and desorption on adsorbents such as silica gel and neusilin using supercritical $CO_2$ as mobile phase was investigated. The experiments were performed at 40 °C and in the pressure range from 13 to 30 MPa. Adsorption kinetic lines were determined by analysingbreakthrough curves. Several parameters such as rate of adsorption and desorption, loading, desorbed quantity and diffusion coefficients in solid phase were calculated.Raziskali smo uporabnost postopka superkritične kromatografije (SFC) za separacijo prostih maščobnih kislin (FFA) in trigliceridov. V eksperimentih adsorpcije in desorpcije na silikagel in neusilin smo uporabili oleinsko kislino in sončnično olje, ki je vsebovalo 96 % trigliceridov. Kot mobilno fazo smo uporabili superkritični $CO_2$ pri 40 °C in v območju tlakov od 13 do 30 MPa. Študirali smo kinetiko adsorpcije in desorpcije in določili parametre kot so adsorpcijska in desorpcijska hitrost, adsorbirana in desorbirana množina substance ter difuzijske koeficiente substanc v trdni fazi (adsorbentu)