Biological activity of the strawberry (Fragaria x ananassa L. Duch.) extract

Rak je zelo zapletena bolezen. Ne moremo je opisati tako natančno kot na primer kako nalezljivo bolezen, pri kateri poznamo povzročitelja, način okužbe, bolezenske spremembe in način obrambe mehanizma, pa tudi uspešno zdravljenje in zaščito. Splošno znano je, da lahko uživanje zadostne količine sadja veliko pripomore k zdravemu načinu življenja. Na kar pa je vredno opozoriti, pa je to, da lahko veliko sadja pridelamo doma na vrtu brez tretiranja s fitofarmacevtskimi sredstvi, ki so danes nepogrešljiv kemični produkt za zaviranje škodljivcev. Veliko raziskav je bilo narejenih z jagodami, ker naj bi bile snovi v jagodah uspešen zaviralec rasti rakavih celic. Namen naše diplomske naloge je bil, da na podlagi raziskav oz. poizkusov dokažemo, da je temu res tako. Z rezultati naših raziskav smo ugotovili, da so snovi, predvsem vitamin C, uspešni zaviralci rasti rakavih celic. Poizkus smo delali z jagodnim ekstraktom v etanolu in PBS-u. Oba sta se izkazala za zelo uspešna pri zaščiti celic pred citotoksičnostjo ter pri zaviranju rasti rakavih celic debelega črevesja (CaCo-2).Cancer is a very complex disease. It cannot be described in such detail as for example an infectious disease in which the agent is known, the route of infection, pathological changes and the way the defence mechanism reacts, as well as successful treatment and protection. It is well known that consuming sufficient quantities of fruit greatly contributes to a healthy lifestyle. On which it is worth noting, however, that much fruit can be produced in the garden at home without treatment with pesticides which are today a significant chemical product to suppress the pests. Much research has been made with strawberries, because the substances in strawberries are supposed to be successful inhibitor of the growth of cancer cells. The purpose of our thesis is to prove with surveys and experiments that this is indeed the case. The results of our research show that the substances, especially vitamin C, are effective inhibitors of cancer cell growth. The test was performed with strawberry extract in ethanol and PBS. Both have proved very successful in protecting cells against the cytotoxicity and growth inhibition in colon cancer cells (CaCo-2)