THE MULTIPLICATION OF POTTED AND GARDEN HYDRANGEAS IN IN VITRO CONDITIONS

Na Fakulteti za kmetijstvo in biosistemske vede v Mariboru smo opravili poskus, v katerem smo proučevali možnosti razmnoževanja lončnih in vrtnih hortenzij v tkivni kulturi. Poganjke smo sterilizirali po štirih metodah: z natrijevim hipokloritom 2 min (sterilizacija S1), natrijevim hipokloritom 10 min (sterilizacija S2), z dikloroizocianurno kislino v kombinaciji s srebrovim nitratom 2 min (sterilizacija S3) in z dikloroizocianurno kislino v kombinaciji s srebrovim nitratom 10 min (sterilizacija S4). Kot najboljša se je izkazala sterilizacija S3, s katero smo uspešno sterilizirali 81,82 % poganjkov. Uspešno sterilizirane poganjke smo prenesli na pet različnih gojišč za regeneracijo (G1, G2, G3, G4 in G5), ki so se razlikovala po osnovnem gojišču (MS oz. McCown) in po vsebnosti rastnih regulatorjev. Med petimi uporabljenimi gojišči, se je gojišče G5 (ki je baziralo na McCown) izkazalo kot najboljše in sicer je namnožitveni faktor po petih tednih znašal 1,33, pri ostalih (G1, G2, G3 in G4) pa nismo uspeli namnožiti nobenega poganjka. Dovolj razvite poganjke smo prestavili na dve gojišči za koreninjenje (K1 in K2), ki sta se razlikovali po vsebnosti avksina (IBA ali NAA). Poganjki vrtne hortenzije so bolje koreninili na gojišču K2, ki je vsebovalo 0,5 mg/l NAA, kjer smo dosegli med 50 in 62,50 % ukoreninjenost. Poganjki lončne hortenzije so bolje koreninili na gojišču K1, ki je vsebovalo 0,5 mg/l IBA in smo dosegli med 53,33 in 100 % ukoreninjenost.At the Faculty of Agriculture and Life Sciences of Maribor we made an experiment in which the possibilities of multiplying potted and garden hydrangeas in tissue culture were studied. The shoots were sterilized according to four different methods: with sodium hypochlorite 2 min (sterilization S1), with sodium hypochlorite 10 min (sterilization S2), with dichloroisocyanuric acid in combination with silver nitrate 2 min (sterilization S3) and with dichloroisocyanuric acid in combination with silver nitrate 10 min (sterilization S4). The sterilization S3 with which we managed to successfully sterilize 81,82 % of the shoots proved the best. The successfully sterilized shoots were then transferred to five different nurseries for their regeneration (G1, G2, G3, G4 and G5), which differed according to the basic nursery (MS resp. McCown) as well as according to the growth regulators. Among the five nurseries used, it was the nursery G5 (based on McCown) that showed the best results with a multiplication factor of 1,33 after five weeks, while in the other nurseries (G1, G2, G3 and G4) we did not succeed in multiplying any of the shoots. The sufficiently developed shoots were then transferred to two rooting nurseries (K1 and K2), which differed only in their auxin contents (IBA and NAA). The shoots of garden hydrangeas did better in the nursery K2, which contained 0,5 mg/l NAA and where the rooting capacity reached from 50 to 62,50 %. The shoots of potted hydrangeas succeeded better in the nursery K1, containing 0,5 mg/l IBA and where a rooting capacity from 53,33 to 100 % was reached