Serum metabolite of Puerariae Radix and related flavonoids induce apoptosis in human breast cancer cells

[[abstract]]Flavonoids constitute the largest and most important group of polyphenolic compounds in plants. They are widely distributed in many frequently consumed beverages and food products of plant origin such as fruit, vegetables, wine and tea. Depending on their oxidation state and functional groups, flavonoids are further divided in six subclasses: flavones, flavanones, flavanols, flavonols, isoflavones, and anthocyanidins .Puerarin, daidzein, and genistein isolated from Puerariae Radix (PR), a popular Chinese herb and a food, have antithrombotic, anti-allergic and estrogenic activities. In addition, they decrease blood lipid levels, reduce oxidative stress, lower cancer risk, cardio-protective, and modulate immune responses. Recent studies demonstrated that isoflavones also possessed anti-cancer properties. Therefore, we investigated the effects of serum metabolite of PR and related isoflavones on inducing apoptosis of breast cancer cell lines. We investigated the effect of serum metabolite of PR on the protein expression of apoptosis-associated genes. The results revealed increased activities of Caspase-3, caspase-9, p53, p21, and Bax. The results revealed that increased caspase-3 activities and modification of the cell cycle caused by the Serum metabolite of Puerariae Radix and related isoflavones. In this report, we investigated the effect of flavonoids on the breast cancer cell of growth. In addition, We also evaluated other flavonoids such as on the growth of breast cancer cell. It result that this flavonids can inhibit the growth of breast cancer cell through inducing apoptosis by activation the Foxo3a