Conversion of an AFLP fragment into one dCAPS marker linked to powdery mildew resistance in melon

Resistance to powdery mildew races 1, 2 and 5 has been evaluated in F2/F3 families from a cross between the resistant line ‘TGR-1551’ and the susceptible Spanish cultivar ‘Bola de oro’. Resistance is controlled by two independent genes, one dominant and one recessive, that both confer resistance to races 1, 2 and 5 of Podosphaera xanthii. Bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) technique have been used for the identification of markers linked to powdery mildew resistance. The AFLP marker E38M82 has been converted into a derived cleaved amplified polymorphic sequences (dCAPS) marker. The marker developed was codominant and the estimated distance with the dominant gene for powdery mildew resistance was 5.7 cM.Cucurbitaceae 2008. IX EUCARPIA Meeting. Avignon (France), May 21-24th, 200