Exploring the cell surface : identification and characterization of lipoproteins in Mycoplasma mycoides subsp. mycoides large colony, Mycoplasma mycoides subsp. capri, and Mycoplasma capricolum subsp. capricolum

The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.Title from PDF of title page (University of Missouri--Columbia, viewed on January 5, 2011).Vita.Thesis advisor: Michael Calcutt.Ph. D. University of Missouri-Columbia 2009.The Mycoplasma mycoides cluster is a group of genetically and antigenically related pathogenic bacteria that infect ruminants. The M. mycoides cluster members that are the focus of this discussion are three caprine pathogens: Mycoplasma mycoides subsp. mycoides Large Colony, Mycoplasma mycoides subsp. capri, and Mycoplasma capricolum subsp. capricolum. These organisms are causative agents of contagious agalactia as well as polyarthritis, pneumonia, mastitis, and septicemia. Mycoplasma possess no cell wall, are among the smallest self-replicating organisms identified, and have lost many biochemical pathways, including those necessary to synthesize sterols and amino acids, thus requiring they exist as obligate parasites within the host organism. This close relationship makes the cell surface a critical point of interaction between the bacterium and the host, not only to survive an attack by the host immune system, but also to acquire nutrients necessary for survival of the microbial pathogen. Proteins on the cell surface may be involved in host specificity or disease determination; therefore we compared five regions throughout the mycoplasmal chromosome of four different M. mycoides cluster members, as well as two different strains of M. mycoides subsp. mycoides Large Colony. These loci were found to contain unique putative lipoprotein genes (lipid modified proteins expressed on the cell surface), many of which show the potential to phase-vary. These genes may be useful to differentiate between members of the cluster. Herein is also described the first phase-variable lipoprotein gene in M. mycoides subsp. mycoides Large Colony. How the bacteria in this cluster cause disease is largely unknown; the only virulence factor identified to date has been the production of hydrogen peroxide via the metabolism of glycerol. This dissertation includes the initial characterization of a putative glycerol binding protein, also a lipoprotein, which may play a role in the ability of the mycoplasma cell to produce hydrogen peroxide and cause damage to host tissues. Understanding how such closely related organisms are able to exhibit variable virulence in unique hosts will depend on the characterization of those proteins expressed on the cell surface and their role in the bacterial lifecycle.Includes bibliographical reference