Photoacoustic discrimination of viable and thermally coagulated blood for burn injury imaging

The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.Title from title screen of research.pdf file (viewed on January 11, 2008)Includes bibliographical references.Thesis (M.S.) University of Missouri-Columbia 2007.Dissertations, Academic -- University of Missouri--Columbia -- Biological engineering.Early and accurate determination of burn depth is crucial to monitoring and treatment of the burn wound. One such treatment, surgical excision and grafting, involves removal of necrotic tissue from the wound and replacing it with healthy skin donated from another area of the body. We propose that a photo acoustically obtained depth profile of the burn wound, which delineates the boundary between necrotic tissue and viable tissue, would prove useful for this intervention. A simplified model of a dermal burn wound can be described as a layer of necrotic tissue, containing thermally coagulated blood, atop a layer of inflamed tissue that is characterized by the presence of viable (non-coagulated) blood. Using optical spectroscopy and photo acoustic spectroscopy, we show that it is possible to discriminate between coagulated and non-coagulated blood using a dual-wavelength photo acoustic method and, therefore, discriminate between the two layer types. A blood vessel phantom study confirmed the feasibility of this dual-wavelength photo acoustic technique. Finally, since little is known about the optical properties of thermally coagulated blood, we sought out to elucidate them. A novel photo acoustic method was used to derive the optical absorption coefficient, [mu]a, of thermally coagulated blood over the wavelength range from 580 to 700 nm. Additionally, we performed a linear regression on the 580 to 700 nm absorption spectrum and extrapolated it out to 500 nm, creating a theoretical 500 to 700 nm absorption spectrum for thermally coagulated blood