The European project HIALINE (Health Impacts of Airborne Allergen Information Network): results of pollen and allergen of Betula monitoring in Parma (2009)

Introduction. Exposure to allergens is pivotal in determining sensitization and allergic symptoms in individuals. Pollen grain counts in ambient air have traditionally been assessed to estimate airborne allergen exposure. However, the exact allergen content in ambient air is unknown. HIALINE therefore monitored atmospheric concentrations of Betula, Poaceae and Olea pollen grains and matched their major allergens Bet v1, Phl p5 and Ole e1 across Europe. Monitoring the allergens themselves together with pollen in ambient air might be an improvement in allergen exposure assessment. New knowledge through the use of new experimental approaches in the field of aerobiological monitoring will enable better in the prevention and clinical management of pollinosis. In order to disseminate the knowledge of the project we present the results of first year of birch pollen grains and the matched major pollen allergen Bet v1 monitored in Parma (UNIPR), Italy with a short reference to the results obtained by the other participants and about developing models of dissemination and forecasts of pollen and allergens. Materials and methods. The pollen was sampled by a Hirst pollen trap. Allergens was collected with a CHEMVOL® high-volume cascade impactor, extracted from pollen and quantified by ELISA. Antibodies for analysis of Bet v1 are delivered by the industrial partner in this project. Quality control has been carried out for the monitoring pollen activities and allergen concentrations. Results. The project has highlighted that it is possible also to measure pollen allergen in ambient air in different European areas. The results obtained from the center of Parma and other European partners have highlighted the different allergenic powers of pollen of Betula, in different geographical areas. Moreover, daily in each area the allergenic power of pollen grains was very variable. Discussion. HIALINE has been a very important project to understand the mechanisms of sensitization, clinical management of pollinosis and to improve immunotherapy towards a tailored immunotherapy. The results of the project will help medical doctors, authorities and patients, to better manage the different aspects related to pollinosis