Effect of Canola oil enrichment with microconsituants against metabolic disorders

Aim/hypothesis: Insulin resistance (IR) favors the progression of metabolicsyndrome (MetS) and increases the risk of type2 diabetes. IR results from metabolic dysfunctions,oxidative stress and inflammation caused by ectopic fat depots. We studied the effect of canola oil enriched with micronutrients naturally present in canola seed on IR and MetS during a high fat (HF)-challenge]. [br/] Research design and Methods: Rats were fed with a HF diet containing 30% of lipids, mainly derived from palm oil (diet P) or from a mixture of palm and canola oils (60:40, diet C). In 2 groups of animals, diet C was further supplemented with alpha tocopherol, CoQ with or without Canolol (diets CMC & CM respectively). Insulin sensitivity and glucose tolerance were assessed after 10 weeks. Tissue sampling was carried at sacrifice for gene expression analysis (RT-qPCR), protein expression level (Western-Blot), fatty acid composition (Gas Chromatography). HF animals were compared to control animals receiving a standard diet. ANOVA was performed for statistical analysis (followed by adjusted Fisher post-hoc test when p