Isolation and identification of dye degrading bacteria from the Textile Sludge (Soil) sample

This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2014.Cataloged from PDF version of thesis.Includes bibliographical references (page 25-27).Textile industry is one amongst the most chemically intensive industries on the face of the earth and the major contaminator of potable water. It produces huge amounts of different complex chemical substances as a part of unused materials that includes dye in the form of wastewater throughout various stages of textile processing. The direct discharge of this wastewater into surroundings affects its ecological status by inflicting various undesirable changes. Bacteria isolated from the textile sludge (soil) sample and inoculated into screening media containing different types of textile dye in aerobic condition to attain the biodegradability of the dye. Within 24 hours of incubation, this strain proved to be a good decolorizer of the dye. Most preferable pH and temperature for dye degradation were pH 7-7.8 and 29-32 °c under aerobic condition. Various nutrient sources such as sodium chloride, beef extract, yeast extract, or peptone strongly boost the decolorization / degradation process and the bacterial efficiency. All the samples showed positive results for the decolorization of the dyes. Under aerobic condition, color of all the dyes reduced by the bacterial biomass that leads to biodegradation.Chinmay PramanikB. Microbiolog