Exploring the Enzymatic Processes of ADA DNA Repair Protein Utilizing the Synthetic Modelling Approach

Ada DNA repair protein is a thiol-alkylating enzyme present within many organisms. The enzyme’s ability to remove alkyl groups from damaged DNA aides in supporting the proper function of DNA and inhibits the growth of certain cancers. The realization of Ada DNA repair protein’s importance has warranted studies directed at obtaining a better understanding of its alkyl-related enzymatic processes. To further study Ada DNA repair protein, synthetic complexes which mimic the enzyme’s active site will be synthesized and their reactivity in a series of crossover reactions will be performed. The synthetic models utilized will consist of zinc-thiolate complexes, as preliminary research has shown the functionality of such complexes is effective in imitating Ada DNA repair protein. We will present results of reactivity and crossover experiments using our synthetic models