A simple and highly efficient cloning method that employs PCR to directly create a fusion between insert and vector

[[abstract]]We have developed a very simple procedure for gene cloning using compound primers. This approach permits the insertion of a DNA fragment in a single step using a PCR-based cloning protocol, which employs annealing rather than ligation to create the recombinant in the plasmid. This method has been tested successfully to clone a Phalaenopsis gene coding for P450. A potential application of this protocol for constructing a cDNA library is also proposed.[[fileno]]2050144010025[[department]]生科