Protein micro arrays immobilized by mu-stamps and -protein wells on PhastGel (R) pad

[[abstract]]This paper reports a novel stamping system, employing mu-stamps and -protein wells to simultaneously transfer proteins onto an array without de-naturalization, cross-contamination, and de-attachment of the proteins. The mu-stamps and -protein wells were successfully fabricated by micro machining and micro molding process. The effect of surface properties of eta-stamp on micro printing has been studied, and results demonstrated the feasibility of printing protein arrays with spot-size of 350 m square and pitch of 100 m. Testing results show that each stamped protein sample can be clearly identified with uniform deposition, and lasts for 6 h under water washing without appreciable de-attachment. This method may be used to transfer numerous different protein samples with the help of pre-filled mu-protein wells. (C) 2002 Elsevier Science B.V. All rights reserved.[[fileno]]2060112010010[[department]]工程與系統科學