Functional Co-substituted Poly[(amino acid ester)phosphazene] Biomaterials

The development of new and improved biomaterials is essential for tissue engineering and regenerative medicine applications. Amino acid-based polyphosphazenes are being explored as scaffold materials for tissue engineering applications due to their non-toxic degradation products and tunable material properties. This work focuses on the synthesis of non-functional and novel functional poly[(amino acid ester)phosphazene]s using a facile method of thermal ring opening polymerization followed by one-pot room temperature substitution. The family of polyphosphazenes developed in this work is based on L-alanine (PNEAs), L-phenylalanine (PNEFs), and L-methionine (PNEMs) with L-glutamic acid imparting the functionality. Characterization of these materials demonstrated that the one-pot substitution was successful in developing mono- and co-substituted poly[(amino acid ester)phosphazene]s. Cytotoxicity studies on two-dimensional films showed these materials to be compatible with NIH-3T3 fibroblasts over the five-day study. The PNEFs also showed significantly enhanced cell viability over tissue culture polystyrene at day 1 (