Supramolecular protection from the enzymatic tyrosine phosphorylation in a polypeptide

Here we report two new artificial pseudopeptidic cages that bind the EYE peptide epitope in pure water at physiological pH (as studied by fluorescence and NMR spectroscopies). The supramolecular complexation of the Tyr residues efficiently precludes their subsequent PTK-catalysed phosphorylation. Our results show a supramolecular modulation of the PTK activity by competitive substrate caging.This work was supported by MINECO/FEDER (CTQ2012-38543-C03, CTQ2015-68429-R and CTQ2015-70117-R projects) and Generalitat de Catalunya (AGAUR, 2014 SGR 231)