Systematic analysis of cross-regulatory profiles in two-component signal transduction systems among closely related bacterial pathogens

研究代表者publisher研究種目：若手研究(A); 研究期間：2011～2014; 課題番号：23688013; 研究分野：分子遺伝学; 科研費の分科・細目：研究成果の概要（和文): 細菌の病原性調節や薬剤耐性遺伝子発現調節等に関わる二成分制御系間のクロスレギュレーションの多様性を解明すべく腸内細菌科に属するサルモネラ菌などにおいて体系的・統計的解析を実施した. 各細菌にコードされる全二成分制御系欠損株の構築等を行い, 相互ネットワーク解析に使用する遺伝ストックとした. 高次ネットワーク形成において二成分制御系間の上下関係をターゲット遺伝子のプロモーター/レポーターシステムを用いた測定により分析することで, 各細菌において「クロスレギュレーション・マップ」を作成した. 本課題の関連研究成果として, 顕著な研究成果を得た. 研究成果の概要（英文）: Two-component system (TCS) is a predominant mode of signal transduction in bacteria. Recent progresses have been drawing detailed pictures of their regulatory networks and evolutional traces. Yet, systematic experimental analyses across species and their statistics are quite challenging. Here, we aimed to analyze cross-regulatory profiles in TCSs of Salmonella enterica and its closely related bacteria by constructing approximately 30 TCS mutant strains for each species. In order to detect novel cross-regulatory phenomena in a quantitative manner, a representative gene promoter, which is directly regulated by a given TCS, was selected for each TCS and cloned into a promoter-less luciferase (lux) reporter/ broad range conjugation plasmid. By mating and testing epistasis in TCSs, we compare extended "TCSs cross-regulatory profiling maps" across species in details. From a certain aspect of these relating analyses, novel TCS regulatory mechanisms were established lately