Survey of xenobiotic-metabolizing cytochrome P450 homologues in higher plant species

継続後誌:近畿大学先端技術総合研究所紀要= Memoirs of Institute of Advanced Technology, Kinki UniversityDNA homologues of rat cytochrome P450 1A 1 (CYP 1A 1) was surveyed by Southern analysis in a total of 98 genotypes consisting of 40 families from higher plant from pteropsida, gymnospermae and angiospermae. Southern analysis was conducted on total genomic DNA digested with HindIII or EcoRI using rat CYP1A1cDNA clone as the probe. Positive signals were detected in 55 and 52 species following digestion with HindIII or EcoRI, respectively. Most of these signals presented as a single band in each plant, and as many as seven bands were detected in a tetraploid potato cultivar. Using potato, fig and green perilla, Southern analysis with 17 restriction enzymes lacking restriction sites in CYP1A1 cDNA were conducted to determine the copy number of CYP1A1 homologues in higher plants. It was speculated that the existence of a few copies of the DNA homologues to CYP1A1 in higher plants. Our results indicated that DNA homologues of CYP1A1 exist widely throughout the plant kingdom with maintaining high homology to those of mammalian