Internalization of 125I-insulin by IM-9 cultured human lymphocytes: a comparison between A14-monoiodo-pork and biosynthetic human insulin

Human insulin synthesized from A- and B-chains separately produced in Escherichia coli from cloned genes was characterized by examining its interaction biochemically and morphologically with IM-9 cultured human lymphocytes. Biosynthetic human insulin (BHI) behaves similarly to pork insulin with respect both to its binding properties and to the rate and magnitude at which it is internalized by the cells