Histological evaluation of contaminated furcal perforation in dog’s teeth repaired by MTA with or without internal matrix.

Aim: To investigate the clinical, radiographic and histologic healing response of experimentally induced furcal perforations in dogs' teeth contaminated with saliva and repaired with MTA with or without artificial f1oor (calcium sulfate). Results were compared to amalgam.Methodology: Seventy-two mandibular and maxillary premolar teeth of 9 beagle's dogs were used. The pulp extirpation was done, the root canals were cleaned and filled with gutta-percha and AH26 under aseptic condition. Furcal perforations were created in 66 teeth using round bur # 4. The perforations were left open to salivary contamination for 4 weeks. The teeth were randomly divided into 6 experimental groups. Group 1 (6 teeth) without perforations served as negative controls, group 2 (6 teeth) with perforation served as positive control, group 3 (15 teeth) the perforations were repaired with MTA alone, and group 4 (15 teeth) with calcium sulfate under MTA, group 5 (15 teeth) the perforations were repaired with amalgam alone, and group 6 (15 teeth) with calcium sulfate under amalgam. Pulp chambers of all experimental teeth were filled with same material used to repair the perforation. Clinical and radiographic examinations were done before and after repair. Animals were sacrificed after 4 months. The teeth and the surrounding structure were processed for light microscopic examination. Results: The clinical, radiographic, and histological findings showed that the MTA alone has better success rate compared to the other groups. Conclusions: The use of calcium sulfate as an internal matrix under MTA or amalgam for repair of delayed furcal perforation did prevent material extrusion but did not enhance the healing process.King Saud Universit