Chemiluminescent determination of pyridoxine hydrochloride in pharmaceutical samples using flow injection

Accurate determination of water-soluble vitamins is very important in the food and pharmaceutical industries.A chemiluminescent method using flow injection is described for the determination of pyridoxine hydrochloride. Its detection limit, linearity and reproducibility were examined. The method is based on the enhancing effect of pyridoxine hydrochloride on the chemiluminescence generated by the oxidation of luminol with hydrogen peroxide in aqueous potassium hydroxide and sodium oxalate. The proposed method is simple and inexpensive. The chemiluminescence intensity is a linear function of pyridoxine hydrochloride concentration over the range 10–250 mg ml 1 with a detection limit of 6 mg ml 1. The applicability of the method was demonstrated by the determination of pyridoxine hydrochloride in different tablet formulations and some dietary sources