Role of the glycoproteins in the production of Sendai virus particles

Previous studies have shown the importance of the Glycoproteins during Sendai virus particle (VP) production. My PhD thesis aims at describing in details the involvement of the F and HN proteins in the process of SeV particle formation. This study performed exclusively in the context of rSeV infections includes: -Identification of the mechanism of action of the F cytoplasmic tail motif, TYTLE, during assembly and budding and its potential interaction with virus proteins and/or cellular machinery. -Determination of HN domains and residues, including the SYWST motif, needed for its uptake into virion. -Global understanding of the molecular mechanism of assembly and budding via the consolidation of outcomes from the two first studies and some additional data. In the end, this thesis work will allow proposing a model for dynamic SeV assembly leading to precursor complex formation and finally virus egress during the last steps of the infection