Among the biofuel production processes using different substrates, the biogas generation process is one of the simplest. Compared with bioethanol or biodiesel production processes, anaerobic digestion is a process where all theorganic matter (carbohydrates, lipids and proteins) can be biologically degradedfor methane production. Biological degradations of those polymeric substrates are carried out by several enzymes during the first stage of hydrolysis. Nevertheless, due to the substrates physical state, microbial degradation is frequently hampered. Substrate pretreatment enhances the performance of anaerobic digestion by improving the bioavailability of the substrate for anaerobic digestion and thus accelerating the hydrolysis step. Approp...