Cloning Ezh1: First Steps Toward an Analysis of Ezh1 Function

Ezh1, a protein involved in the regulation of skeletal muscle differentiation, was cloned into a vector and analyzed. As a member of the polycomb repressive complex 2 (PRC2), Ezh1 has been shown to be an early catalyst in recruiting the complex to cells. Cancer cells, however, exhibit an overexpression of PRC2, so an excess of the protein in a cell can ultimately lead to complications and uncontrollable proliferation. After ligating and transforming the E. coli cells with the desired vector and insert, the plasmids were extracted. Western gel electrophoresis later proved that the protein was successfully expressed in the cell, which enables further experimentation on the protein to be performed