Immunogold labelling of SRPK2ΔS1/CpWT capsid complex.

Immuno-labeling assay to detect actin, myosin, ferritin 2, and ATP synthase in IDVs.

Chin-Yuan Hsu (25618)
Yu-Pei Chan (352666)

February 2013

(A–D) The DIC image of trophocytes observed using CM. Scale bar, 5 µm. (E–H) Immunofluorescent de...

Immunoprecipitation of MRC-5 V2 proteins with anti-calumenin antiserum.

Gry Aune Westergaard Hansen (768292)
Maja Ludvigsen (484778)
Christian Jacobsen (768293)
Claudia Cangemi (768294)
Lars Melholt Rasmussen (497181)
Henrik Vorum (414798)
Bent Honoré (484780)

July 2015

(A) 35S-labelled MRC-5 V2 proteins were separated by 2D-PAGE, blotted onto a membrane ...

Colocalization of PLP and GFP-MAL2 with actin cytoskeleton.

Raquel Bello-Morales (351962)
Marta Pérez-Hernández (351963)
María Teresa Rejas (351964)
Fuencisla Matesanz (166725)
Antonio Alcina (166754)
José Antonio López-Guerrero (351965)

February 2013

GFP-MAL2/HOG cells cultured in DM at 37°C for 2 days were fixed and processed for confocal triple...

Size-exclusion chromatography analysis of SRPK2-CpWT complex.

Ryan Pak Hong YIP (17921764)
Doris Ching Ying Kwok (17921767)
Louis Tung Faat Lai (6919313)
Siu-Ming Ho (17921770)
Ivan Chun Kit Wong (17921773)
Chi-Ping Chan (8947316)
Wilson Chun Yu Lau (8377197)
Jacky Chi Ki Ngo (5950379)

February 2024

(A) Size-exclusion chromatograph of CpWT after the removal of GuHCl by dialysis. All CpWT assembled ...

Analysis of 32P labelled capsids.

Birgit Rabe (254176)
Mildred Delaleau (370916)
Andreas Bischof (370917)
Michael Foss (254173)
Irina Sominskaya (370918)
Paul Pumpens (28426)
Christian Cazenave (370919)
Michel Castroviejo (7333)
Michael Kann (119825)

February 2013

A. Labelling of virion-derived capsids (P-Mat-C) using the endogenous protein kinase (left) and <...

Immunogold labelling of ubiquitylated proteins in the nacreous layer (A and B) and the prismatic layer (C and D).

Dong Fang (168743)
Cong Pan (168752)
Huijuan Lin (168759)
Ya Lin (168767)
Guangrui Xu (168774)
Guiyou Zhang (168777)
Hongzhong Wang (168785)
Liping Xie (168792)
Rongqing Zhang (168798)

April 2012

The complexes formed by the antigen–1st antibody–2nd gold-labeled antibody were identified as tin...

SV40 VP1 major capsid protein in its self-assembled form allows VP1 pentamers to coat various types of artificial beads in vitro regardless of their sizes and shapes

Kawano, Masaaki
Doi, Koji
Fukuda, Hajime
Kita, Yoshinori
Imai, Kensuke
Inoue, Takamasa
Enomoto, Teruya
Matsui, Masanori
Hatakeyama, Mamoru
Yamaguchi, Yuki
Handa, Hiroshi

March 2015

AbstractThe icosahedral capsid structure of simian virus 40 (diameter, 45nm) consists of 72 pentamer...

CHIP forms a protein complex with BAG5.

Lorraine V. Kalia (355943)
Suneil K. Kalia (355944)
Hien Chau (355945)
Andres M. Lozano (355946)
Bradley T. Hyman (257165)
Pamela J. McLean (355947)

February 2013

(A) Immunoprecipitations with anti-FLAG antibodies were performed from lysates of H4 cells transf...

HSPC111 resides in high molecular weight protein complexes in the nucleolus

Alison J Butt (48657)
C Marcelo Sergio (48658)
Claire K Inman (48659)
Luke R Anderson (48660)
Catriona M McNeil (48661)
Amanda J Russell (48662)
Marco Nousch (48663)
Thomas Preiss (3188)
Andrew V Biankin (48664)
Robert L Sutherland (48665)
Elizabeth A Musgrove (48666)

December 2011

Detection of endogenous and tagged HSPC111 by indirect immunofluorescence. Upper panels: Immunostain...

Analysis of capsids by Nycodenz gradient ultracentrifugation and electron microcopy.

Birgit Rabe (254176)
Mildred Delaleau (370916)
Andreas Bischof (370917)
Michael Foss (254173)
Irina Sominskaya (370918)
Paul Pumpens (28426)
Christian Cazenave (370919)
Michel Castroviejo (7333)
Michael Kann (119825)

February 2013

A–C: Nycodenz gradient ultracentrifugation using 50 ng of capsids. The graphs show the fractions ...

Preliminary analyses with antibody and immuno-AuNPs.

Subash C. B. Gopinath (10681)
Koichi Awazu (433069)
Makoto Fujimaki (433070)
Kazufumi Shimizu (284611)
Takayuki Shima (433071)

July 2013

(a) Dot-blot analyses with immuno-AuNPs. Different concentrations of immuno-AuNPs were spotted on...

Identification of Nck1 as a component of the cytoplasmic capping complex.

Chandrama Mukherjee (95107)
Baskar Bakthavachalu (618180)
Daniel R. Schoenberg (463062)

August 2014

(A) Cytoplasmic extract from cells expressing bio-cCE was separated on a 10%–50% glycerol gradien...

Identification of amino acids between residues VP1 185 and 188 required for capsid precursor processing The P1-2A precursors (wt or having alanine substitutions between VP1 185 and VP1 188) were expressed alone or in the presence of 3Cpro and analyzed by immunoblotting (as above).

Thea Kristensen (4489387)
Graham J. Belsham (116715)

January 2019

The structural proteins corresponding to the different products are indicated on the right of the fi...

Immune precipitation of light and heavy capsid fractions from Nycodenz gradients after nuclear import of Mat-C.

Birgit Rabe (254176)
Mildred Delaleau (370916)
Andreas Bischof (370917)
Michael Foss (254173)
Irina Sominskaya (370918)
Paul Pumpens (28426)
Christian Cazenave (370919)
Michel Castroviejo (7333)
Michael Kann (119825)

February 2013

Phosphoimager scan after SDS PAGE. D: precipitation by DAKO Ab, F: precipitation by FAb3105, m: m...

Immunogold labeling on cryo-sections.

Elia Diestra (368640)
Bastien Cayrol (368642)
Véronique Arluison (25027)
Cristina Risco (368646)

February 2013

(A) and (B) Double immunogold labeling of hfq− E. coli cells expressing Hfq-MT ...

Immuno-labeling assay to detect actin, myosin, ferritin 2, and ATP synthase in IDVs.

Chin-Yuan Hsu (25618)
Yu-Pei Chan (352666)

February 2013

(A–D) The DIC image of trophocytes observed using CM. Scale bar, 5 µm. (E–H) Immunofluorescent de...

Immunoprecipitation of MRC-5 V2 proteins with anti-calumenin antiserum.

Gry Aune Westergaard Hansen (768292)
Maja Ludvigsen (484778)
Christian Jacobsen (768293)
Claudia Cangemi (768294)
Lars Melholt Rasmussen (497181)
Henrik Vorum (414798)
Bent Honoré (484780)

July 2015

(A) 35S-labelled MRC-5 V2 proteins were separated by 2D-PAGE, blotted onto a membrane ...

Colocalization of PLP and GFP-MAL2 with actin cytoskeleton.

Raquel Bello-Morales (351962)
Marta Pérez-Hernández (351963)
María Teresa Rejas (351964)
Fuencisla Matesanz (166725)
Antonio Alcina (166754)
José Antonio López-Guerrero (351965)

February 2013

GFP-MAL2/HOG cells cultured in DM at 37°C for 2 days were fixed and processed for confocal triple...

Size-exclusion chromatography analysis of SRPK2-CpWT complex.

Ryan Pak Hong YIP (17921764)
Doris Ching Ying Kwok (17921767)
Louis Tung Faat Lai (6919313)
Siu-Ming Ho (17921770)
Ivan Chun Kit Wong (17921773)
Chi-Ping Chan (8947316)
Wilson Chun Yu Lau (8377197)
Jacky Chi Ki Ngo (5950379)

February 2024

(A) Size-exclusion chromatograph of CpWT after the removal of GuHCl by dialysis. All CpWT assembled ...

Analysis of 32P labelled capsids.

Birgit Rabe (254176)
Mildred Delaleau (370916)
Andreas Bischof (370917)
Michael Foss (254173)
Irina Sominskaya (370918)
Paul Pumpens (28426)
Christian Cazenave (370919)
Michel Castroviejo (7333)
Michael Kann (119825)

February 2013

A. Labelling of virion-derived capsids (P-Mat-C) using the endogenous protein kinase (left) and <...

Immunogold labelling of ubiquitylated proteins in the nacreous layer (A and B) and the prismatic layer (C and D).

Dong Fang (168743)
Cong Pan (168752)
Huijuan Lin (168759)
Ya Lin (168767)
Guangrui Xu (168774)
Guiyou Zhang (168777)
Hongzhong Wang (168785)
Liping Xie (168792)
Rongqing Zhang (168798)

April 2012

The complexes formed by the antigen–1st antibody–2nd gold-labeled antibody were identified as tin...

SV40 VP1 major capsid protein in its self-assembled form allows VP1 pentamers to coat various types of artificial beads in vitro regardless of their sizes and shapes

Kawano, Masaaki
Doi, Koji
Fukuda, Hajime
Kita, Yoshinori
Imai, Kensuke
Inoue, Takamasa
Enomoto, Teruya
Matsui, Masanori
Hatakeyama, Mamoru
Yamaguchi, Yuki
Handa, Hiroshi

March 2015

AbstractThe icosahedral capsid structure of simian virus 40 (diameter, 45nm) consists of 72 pentamer...

CHIP forms a protein complex with BAG5.

Lorraine V. Kalia (355943)
Suneil K. Kalia (355944)
Hien Chau (355945)
Andres M. Lozano (355946)
Bradley T. Hyman (257165)
Pamela J. McLean (355947)

February 2013

(A) Immunoprecipitations with anti-FLAG antibodies were performed from lysates of H4 cells transf...

HSPC111 resides in high molecular weight protein complexes in the nucleolus

Alison J Butt (48657)
C Marcelo Sergio (48658)
Claire K Inman (48659)
Luke R Anderson (48660)
Catriona M McNeil (48661)
Amanda J Russell (48662)
Marco Nousch (48663)
Thomas Preiss (3188)
Andrew V Biankin (48664)
Robert L Sutherland (48665)
Elizabeth A Musgrove (48666)

December 2011

Detection of endogenous and tagged HSPC111 by indirect immunofluorescence. Upper panels: Immunostain...

Analysis of capsids by Nycodenz gradient ultracentrifugation and electron microcopy.

Birgit Rabe (254176)
Mildred Delaleau (370916)
Andreas Bischof (370917)
Michael Foss (254173)
Irina Sominskaya (370918)
Paul Pumpens (28426)
Christian Cazenave (370919)
Michel Castroviejo (7333)
Michael Kann (119825)

February 2013

A–C: Nycodenz gradient ultracentrifugation using 50 ng of capsids. The graphs show the fractions ...

Preliminary analyses with antibody and immuno-AuNPs.

Subash C. B. Gopinath (10681)
Koichi Awazu (433069)
Makoto Fujimaki (433070)
Kazufumi Shimizu (284611)
Takayuki Shima (433071)

July 2013

(a) Dot-blot analyses with immuno-AuNPs. Different concentrations of immuno-AuNPs were spotted on...

Identification of Nck1 as a component of the cytoplasmic capping complex.

Chandrama Mukherjee (95107)
Baskar Bakthavachalu (618180)
Daniel R. Schoenberg (463062)

August 2014

(A) Cytoplasmic extract from cells expressing bio-cCE was separated on a 10%–50% glycerol gradien...

Identification of amino acids between residues VP1 185 and 188 required for capsid precursor processing The P1-2A precursors (wt or having alanine substitutions between VP1 185 and VP1 188) were expressed alone or in the presence of 3Cpro and analyzed by immunoblotting (as above).

Thea Kristensen (4489387)
Graham J. Belsham (116715)

January 2019

The structural proteins corresponding to the different products are indicated on the right of the fi...

Immune precipitation of light and heavy capsid fractions from Nycodenz gradients after nuclear import of Mat-C.

Birgit Rabe (254176)
Mildred Delaleau (370916)
Andreas Bischof (370917)
Michael Foss (254173)
Irina Sominskaya (370918)
Paul Pumpens (28426)
Christian Cazenave (370919)
Michel Castroviejo (7333)
Michael Kann (119825)

February 2013

Phosphoimager scan after SDS PAGE. D: precipitation by DAKO Ab, F: precipitation by FAb3105, m: m...

Immunogold labeling on cryo-sections.

Elia Diestra (368640)
Bastien Cayrol (368642)
Véronique Arluison (25027)
Cristina Risco (368646)

February 2013

(A) and (B) Double immunogold labeling of hfq− E. coli cells expressing Hfq-MT ...

Immuno-labeling assay to detect actin, myosin, ferritin 2, and ATP synthase in IDVs.

Chin-Yuan Hsu (25618)
Yu-Pei Chan (352666)

February 2013

(A–D) The DIC image of trophocytes observed using CM. Scale bar, 5 µm. (E–H) Immunofluorescent de...

Immunoprecipitation of MRC-5 V2 proteins with anti-calumenin antiserum.

Gry Aune Westergaard Hansen (768292)
Maja Ludvigsen (484778)
Christian Jacobsen (768293)
Claudia Cangemi (768294)
Lars Melholt Rasmussen (497181)
Henrik Vorum (414798)
Bent Honoré (484780)

July 2015

(A) 35S-labelled MRC-5 V2 proteins were separated by 2D-PAGE, blotted onto a membrane ...

Colocalization of PLP and GFP-MAL2 with actin cytoskeleton.

Raquel Bello-Morales (351962)
Marta Pérez-Hernández (351963)
María Teresa Rejas (351964)
Fuencisla Matesanz (166725)
Antonio Alcina (166754)
José Antonio López-Guerrero (351965)

February 2013

GFP-MAL2/HOG cells cultured in DM at 37°C for 2 days were fixed and processed for confocal triple...

Immunogold labelling of SRPK2ΔS1/CpWT capsid complex.

Abstract

Extracted data

Immunogold labelling of SRPK2ΔS1/CpWT capsid complex.

Abstract

Extracted data

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