Overall cryo-EM structures of CpWT capsid and the complex with SRPK2ΔS1.

(A-B) 3D reconstruction and the cutaway views of (A) the apo-capsid and (B) the complex of SRPK2ΔS1 and CpWT capsid. Density maps are colored according to the local resolutions. 2-fold, 3-fold, and 5-fold axes are denoted by an oval, a triangle, and a pentagon respectively. The scale bars represent 10 nm. The corresponding FSC curves are shown on the right. (C) The crystal structure of the HBV core protein (PDB: 1QGT) is fitted into the density maps of the apo-capsid and SRPK2ΔS1/CpWT. The N-termini of four core protein monomers were indicated by red arrows. Extra densities attributed by the His-tags of CpWT are indicated. No extra unaccounted density is observed. (D) Overall cryo-EM 3D reconstruction and resolution assessment of the focus-refined sub-particle. The 3D reconstruction is colored by the local resolution. Scale bar represents 5 nm. The FSC curve is shown on the right. (TIF)