Processing of cryo-EM images of CpWT capsid and the complex with SRPK2ΔS1.

(A) Representative micrograph of SRPK2ΔS1/CpWT capsid complex with a scale bar of 100 nm (left). White arrowheads indicate the protruding ends on the capsid surface. Reference-free 2D class averages of particles extracted from micrographs (right). 2D class averages were divided into two groups: capsids with extra densities on surface, which are indicated by red arrowheads, and capsids without extra surface densities. Particles with extra surface densities were used for the subsequent 3D classification. The box size is 512 Å. (B) Representative micrograph of CpWT capsid with a scale bar of 100 nm (left). Reference-free 2D class averages of particles extracted from micrographs (right). The box size is 480 Å. (TIF)