Anti-tubulin and phalloidin staining of papillae in whole-mounted tissues.

Cilia clusters on the lobules are green (anti-tubulin). Muscle is red (phalloidin). A-C: Antibody and phalloidin penetrated only the superficial level of the tissues, probably through the clusters, revealing the clusters of sensory cilia in the lobules (arrowheads) and the adjacent muscle fibers (arrows). Finer cilia surround the central bundles. Muscle fibers lie immediately beneath the cilia clusters. D: A confocal section of C showing off the glomerular tangles below the cilia (arrowhead) and muscle fibers (arrows). Scale bars A, 200 μm; B-D, 50 μm.