TXNIP targets glycolysis and the pentose phosphate pathway.

Heat map analysis of LC-MS metabolomics. The diagram depicts glycolysis (green), pentose-phosphate pathway (yellow), and TCA cycle (blue). The GLUT is denoted in red. HEK293T cells were transduced with lentiviruses carrying vector control or TXNIP gene and were collected 48 hrs post transduction and prepared for LC-MS metabolomics. In TXNIP expressing cells, metabolites that increased were displayed in red and metabolites that decreased were displayed in blue. The heat map was created using MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/MetaboAnalyst/ModuleView.xhtml) as described in Materials and methods. Glycose-6P, Glucose 6-phosphate; Erythrose 4P, Erythrose 4-phoshate; Ribulose 5P, Ribulose 5-phosphate; Fructose 6P, Fructose 6-phosphate; Fructose 1,6-biP, Fructose 1,6-bisphosphate; Glyceraldehyde 3P, Glyceraldehyde 3-phosphate; Glycerate P, Glycerate phosphate; PYR, pyruvate; CIT, citrate; αKG, α-ketoglutarate; SUC, succinate; MAL, malate; FUM, fumarate. Figure was created by BioRender.com. See also S1 Fig in S1 File.