Stress tolerance of complemented strains.

(A, B) Samples were serially diluted, plated in duplicate on TY Tau plates and incubated either in anaerobiosis or in hypoxia at (A) 1% O2 or (B) 0.1% O2 for 64 h. Survival was then normalized by doing the ratio of the mutant vs the WT. (C, D) Samples were serially diluted and plated on TY and on (C) TY + DEA NONOate 750 μM or (D) TY + HClO 0.1% and incubated for 24 h. Mean and SD are shown. Experiments were performed in 5 biological replicates. For all assays, one-way ANOVA were performed followed by Dunett’s multiple comparison tests. *: p-value (TIF)