The effect of leguminous leaf meal substitution as a tannin source in complete feed on Rumen Degradable Protein (RDP), Rumen Undegradable Protein (UDP), and Digestible Energy (DE) using in vitro analysis

This study aimed to assess the impact of substituting concentrate with leguminous leaf meal as a source of tannins in complete feed on Rumen Degradable Protein (RDP), Rumen Undegradable Protein (UDP), and Digestible Energy (DE) content in vitro. The complete feeds were formulated using ingredients such as corn stover, soybean meal, copra meal, cassava meal, rice bran, pollard, molasses, minerals, calliandra meal, gamal meal, and indigofera meal. The experiment included ten treatments, each replicated three times. The treatments were as follows: T1 complete feed (60% corn stover + 40% concentrate), T2 complete feed (60% corn stover + (35% concentrate + 5% calliandra meal), T3 complete feed (60% corn stover + (35 % concentrate) + 5% indigofera meal), complete feed T4 (60% corn stover + (35% concentrate + 5% gamal meal), T5 complete feed (60% corn stover + (30% concentrate + 10% calliandra meal), T6 complete feed (60% corn stover + (30% concentrate + 10% indigofera meal), complete feed T7 (60% corn stover + (30% concentrate + 10% gamal meal), complete feed T8 (60% corn stover + (25 ) % concentrate + 15% calliandra meal), complete feed T9 (60% corn stover + (25% concentrate + 15% indigofera meal), complete feed T10 (60% corn stover + (25% concentrate + 15% gamal meal). The conclusion of this study reveals that the addition of 15% calliandra meal reduced RDP by 46.56% and increased UDP by 53.44%. Furthermore, the inclusion of 15% gliricidia meal led to an increase in DE value by 3.46 MJ/KgDM