DataSheet_1_Prediction of biomass accumulation and tolerance of wheat seedlings to drought and elevated temperatures using hyperspectral imaging.docx

Early prediction of important agricultural traits in wheat opens up broad prospects for the development of approaches to accelerate the selection of genotypes for further breeding trials. This study is devoted to the search for predictors of biomass accumulation and tolerance of wheat to abiotic stressors. Hyperspectral (HS) and chlorophyll fluorescence (ChlF) parameters were analyzed as predictors under laboratory conditions. The predictive ability of reflectance and normalized difference indices (NDIs), as well as their relationship with parameters of photosynthetic activity, which is a key process influencing organic matter production and crop yields, were analyzed. HS parameters calculated using the wavelengths in Red (R) band and the spectral range next to the red edge (FR-NIR) were found to be correlated with biomass accumulation. The same ranges showed potential for predicting wheat tolerance to elevated temperatures. The relationship of HS predictors with biomass accumulation and heat tolerance were of opposite sign. A number of ChlF parameters also showed statistically significant correlation with biomass accumulation and heat tolerance. A correlation between HS and ChlF parameters, that demonstrated potential for predicting biomass accumulation and tolerance, has been shown. No predictors of drought tolerance were found among the HS and ChlF parameters analyzed.