Female mice performed significantly better than male mice in the Barnes maze.

(A) During the trial day period, there was a significant effect of sex as female mice found the target hole significantly faster than male mice at 3-, 6-, 9-, and 12-months of age (3-Months- F(1, 64) = 6.07, p = 0.016; 6-Months- F(1, 67) = 5.00, p = 0.029; 9-Months- F(1, 64) = 15.45, p = 0.0002; 12-Months- F(1, 62) = 7.78, p = 0.007; repeated measures ANOVA). (B) There was also a significant effect of sex in the 1-day probe test as female mice found the target hole significantly faster than male mice at 6-, 9-, and 12-months of age (6-Months- F(1, 67) = 7.92, p = 0.006; 9-Months- F(1, 65) = 15.89, p = 0.0002; 12-Months- F(1, 62) = 6.86, p = 0.011; ANOVA). (DOCX)