Genotype detection of EMS-induced mutant zmlbd1 in maize, and double mutant lbd16 lbd18 in Arabidopsis.

(A) Schematic representation of EMS-induced mutation site in the exon of ZmLBD1 and alignment of genomic sequence of zmlbd1 mutants with B73, zmlbd1 and control reference sequence to identify genotypes of independent mutant lines (B) Genomic DNA extracted from Col-0, double mutant lbd16 lbd18 and lbd16 lbd18/35S::ZmLBD1 was subjected to PCR amplification with primers specific to LBD16 or LBD18 and the general primers for T-DNA. LP and RP represent the specific left and right primers, respectively, and LB represents the general primer. C1#, C2#, and C3# represent three independent complementary lines. M = DNA marker, 1–24 represents partly independent single clones. (TIF)