Cryo-EM data-processing pipeline for the AAV9P31-Car4 complex.

Overview of the cryo-EM data processing pipeline in RELION [48,49]. (A) Cryo-EM sample data collected in a 300 kV Titan Krios electron microscope with 0.8433 Å per pixel. (B) The micrographs are selected with the CTF estimated maximum resolution (threshold: (C) Particles are automatically picked from micrographs. (D) The particles selected by multiple rounds of 2D classifications. (E) Multiple rounds of 3D classifications with C1 symmetry, after which two classes were selected for the 3D refinement. (F) The AAV9P31-Car4 complex was reconstructed with I1 symmetry after local CTF refinement. The selected classes are colored gray and unselected classes are colored white. (PDF)