Cryo-EM reconstruction of AAV9P31 and AAV9P31-Car4.

(A) The reconstructed cryo-EM map of AAV9P31. The radial distance color scheme is used to indicate the density (140 Å: red). (B) The trimer of AAV9P31 VP3 in the threefold axis is colored by surface. (C) Cartoon representations of (D). The inserted peptide of AAV9P31 is colored bright orange in (B) and (C). (D) Overall cryo-EM map of the AAV9P31-Car4 complex. The radial distance color scheme is used to indicate the density by color (140 Å: red). (E) The trimer of AAV9P31-Car4 in the threefold axis is colored by surface. (F) Cartoon representations of (E). Capsomer A is colored slate blue; capsomer B is colored cyan; capsomer C is colored smudge green; Car4 is colored red (B, C, E, F). The 5-, 3- and 2-fold icosahedral axes of symmetry are indicated with a pentagon, triangles, and an oval, respectively (B, C, E, F).