Survival frequencies rad52Δ and wild type strains using GAL::Cas9. The graph shows the results of 1-tail, 2-tail, tailless as well as a strain where Cas9 cuts both junctions between the repeats and the spacer DNA (2-DSB strain diagrammed in Fig 1B).</p
<p>(A) DSB repair by SSA. YEPR exponentially growing cell cultures of YMV45 derivative strains, carr...
<p>Serial dilutions (ten-fold) of indicated strains (see below) plated on YPAD plates with MMS or wi...
<p>(A) Exponentially growing YEPR cell cultures of JKM139 derivative strains were arrested in G2 wit...
<p>An unrepairable DSB was created in wild-type (yXW1), <i>rad51</i>Δ (ySL306), and <i>rad52</i>Δ (y...
A. Both mismatches and nonhomologous tails reduce SSA. The frequency of DSB-induced SSA was measured...
<p>(<b>A</b>) GCR were measured after exposure to 0.02% MMS, which results in 10% survival rate for ...
Mitotic homologous recombination (HR) stabilises the genome by repairing harmful double stranded DNA...
<p><b>(A, D)</b> Cell growth comparative analyses of <i>tlc1Δ</i> with <i>tlc1Δ rad52Δ</i> (A) and <...
<p>DSB detection was carried out as described in <a href="http://www.plosgenetics.org/article/info:d...
A plasmid containing 200 bp repeats, URA3, and CEN6 was transformed into wild type and rad52Δ strain...
<p>(A) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out on...
The effect of MMR proteins, helicases and recombinases on SSA repair efficiency. GAL::HO and GAL::Ca...
(A). Representative gel image of experiments designed to test how efficiently Rdh54, Rad54NRdh54, an...
<p>(A) Nuclei with RAD51 foci were scored following a DSB at a chromosome-internal locus (Control) o...
The sfiA::lacZ reporter system was to measure SOS induction in mutant strains additional to those sh...
<p>(A) DSB repair by SSA. YEPR exponentially growing cell cultures of YMV45 derivative strains, carr...
<p>Serial dilutions (ten-fold) of indicated strains (see below) plated on YPAD plates with MMS or wi...
<p>(A) Exponentially growing YEPR cell cultures of JKM139 derivative strains were arrested in G2 wit...
<p>An unrepairable DSB was created in wild-type (yXW1), <i>rad51</i>Δ (ySL306), and <i>rad52</i>Δ (y...
A. Both mismatches and nonhomologous tails reduce SSA. The frequency of DSB-induced SSA was measured...
<p>(<b>A</b>) GCR were measured after exposure to 0.02% MMS, which results in 10% survival rate for ...
Mitotic homologous recombination (HR) stabilises the genome by repairing harmful double stranded DNA...
<p><b>(A, D)</b> Cell growth comparative analyses of <i>tlc1Δ</i> with <i>tlc1Δ rad52Δ</i> (A) and <...
<p>DSB detection was carried out as described in <a href="http://www.plosgenetics.org/article/info:d...
A plasmid containing 200 bp repeats, URA3, and CEN6 was transformed into wild type and rad52Δ strain...
<p>(A) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out on...
The effect of MMR proteins, helicases and recombinases on SSA repair efficiency. GAL::HO and GAL::Ca...
(A). Representative gel image of experiments designed to test how efficiently Rdh54, Rad54NRdh54, an...
<p>(A) Nuclei with RAD51 foci were scored following a DSB at a chromosome-internal locus (Control) o...
The sfiA::lacZ reporter system was to measure SOS induction in mutant strains additional to those sh...
<p>(A) DSB repair by SSA. YEPR exponentially growing cell cultures of YMV45 derivative strains, carr...
<p>Serial dilutions (ten-fold) of indicated strains (see below) plated on YPAD plates with MMS or wi...
<p>(A) Exponentially growing YEPR cell cultures of JKM139 derivative strains were arrested in G2 wit...