Sample collection and processing methods.

From each dog, the fecal sample was cut longitudinally in half (A). One half was divided into eight non-homogenized samples, while the other half was divided into 16 homogenized aliquots (B). Four homogenized and non-homogenized samples each were stored at room temperature for 0.5, 4, 8, or 24 h (C). Of the homogenized samples at each room temperature exposure time point, two had RNAlater added (D). Samples were then frozen immediately, or stored in the fridge for 24 h before being transferred to the freezer (E).