Identification and Characterization of lncRNAs in <i>S</i>. <i>japonicum</i>.

(A) The schematic diagram of schistosomes at four-time points and the bioinformatics workflow for lncRNA prediction. See the description in Methods for details. The cartoon mouse was modified from a public domain image by Creazilla (https://creazilla.com/nodes/19261-cartoon-grey-mouse-clipart). (B) Circos plot showing the chromosomal distribution of lncRNAs. The histogram indicates the abundance of lncRNAs(black) and mRNAs(orange) in physical bins of 10 Kb for each chromosome. Lines located in the innermost circle mark the genomic localization of lncRNAs. (C) The classification of lncRNAs into four categories (intergenic, antisense genic, sense genic, and isolated) according to the relationships between lncRNAs and their adjacent mRNA. (D) GC content of lncRNA transcripts (lncRNA), protein-coding sequences (CDS), and intergenic sequences (Intergenic). The respective average values for the GC content of LncRNA, CDS, and Intergenic regions are 35.39%, 36.59%, and 34.83%. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s multiple comparisons, ****, P-value < 0.0001. (E) Lengths distribution of lncRNA and mRNA transcripts. (F) Numbers of exons in lncRNA and mRNA genes.