The Rudiviridae are a family of rod-shaped archaeal viruses with covalently closed, linear double-stranded DNA (dsDNA) genomes. Their replication mechanisms remain obscure, although parallels have been drawn to the Poxviridae and other large cytoplasmic eukaryotic viruses. Here we report that a protein encoded in the 34-kbp genome of the rudivirus SIRV1 is a member of the replication initiator (Rep) superfamily of proteins, which initiate rolling-circle replication (RCR) of diverse viruses and plasmids. We show that SIRV Rep nicks the viral hairpin terminus, forming a covalent adduct between an active-site tyrosine and the 5' end of the DNA, releasing a 3' DNA end as a primer for DNA synthesis. The enzyme can also catalyze the joining react...
Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential r...
Ability to replicate and evolve are two distinct features of all living entities. Duplication of gen...
The RepE protein of the broad host range pAMβ1 plasmid from Gram-positive bacteria is absolutely req...
Rolling circle replication (RCR) is ubiquitously used by cellular and viral systems for genome and p...
ABSTRACT Circular Rep-encoding single-stranded DNA (CRESS-DNA) viruses infect members from all three...
International audienceFor most archaeal viruses, the mechanisms of genome replication are poorly und...
Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in det...
-acting origin of replication and a virus specific Replication protein (Rep) to faithfully carry out...
Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in det...
AbstractInteraction between the adenoassociated virus (AAV) replication proteins, Rep68 and 78, and ...
Geminiviruses primarily encode only few factors, such as replication initiator protein (Rep), and ne...
International audienceGenomic DNA replication is a complex process that involves multiple proteins. ...
The double-stranded DNA genomes of the viruses SIRV1 and SIRV2, which infect the extremely thermophi...
The majority of archaeal viral genes are of unknown function hindering our understanding of the viru...
International audienceThe replication of members of the two circular single-stranded DNA (ssDNA) vir...
Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential r...
Ability to replicate and evolve are two distinct features of all living entities. Duplication of gen...
The RepE protein of the broad host range pAMβ1 plasmid from Gram-positive bacteria is absolutely req...
Rolling circle replication (RCR) is ubiquitously used by cellular and viral systems for genome and p...
ABSTRACT Circular Rep-encoding single-stranded DNA (CRESS-DNA) viruses infect members from all three...
International audienceFor most archaeal viruses, the mechanisms of genome replication are poorly und...
Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in det...
-acting origin of replication and a virus specific Replication protein (Rep) to faithfully carry out...
Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in det...
AbstractInteraction between the adenoassociated virus (AAV) replication proteins, Rep68 and 78, and ...
Geminiviruses primarily encode only few factors, such as replication initiator protein (Rep), and ne...
International audienceGenomic DNA replication is a complex process that involves multiple proteins. ...
The double-stranded DNA genomes of the viruses SIRV1 and SIRV2, which infect the extremely thermophi...
The majority of archaeal viral genes are of unknown function hindering our understanding of the viru...
International audienceThe replication of members of the two circular single-stranded DNA (ssDNA) vir...
Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential r...
Ability to replicate and evolve are two distinct features of all living entities. Duplication of gen...
The RepE protein of the broad host range pAMβ1 plasmid from Gram-positive bacteria is absolutely req...