Properties of a novel potassium current that is active at resting potential in rabbit pulmonary artery smooth muscle cells

1. An outward current (I-K(N)) was identified in rabbit pulmonary artery myocytes, which persisted after Ca2+-activated and ATP-sensitive K+ currents were blocked by TEA (10 mM) and glibenclamide (10 mu M), respectively, and after A-like (I-K(A)) and delayed rectifier (I-K(V)) K+ currents were inactivated by clamping the cell at 0 mV for >10 min. It was found in smooth muscle cells at all levels of the pulmonary arterial tree.2. The relationship between the reversal potential of I-K(N) and the extracellular K+ concentration ([K+](o)) was close to that expected for a K+-selective channel. Deviation from Nernstian behaviour at low [K+](o) could be accounted for by the presence of an accompanying leakage current.3. I-K(N) is voltage gated. It has a low threshold for activation, between -80 and -65 mV, and activates slowly without delay. Activation follows an exponential time course with a time constant of 1.6 s at -60 mV. Deactivation is an order of magnitude faster-than activation, with a time constant of 107 ms at -60 mV.4. I-K(N) showed a similar sensitivity to 4-aminopyridine as I-K(A) and I-K(V), with 49% inhibition at 10 mM. The current was not blocked by 10 mu M quinine, which did inhibit I-K(A) and I-K(V), by 51 and 47%, respectively.5. Activation of I-K(N) was detected at potentials close to the resting membrane potential of pulmonary artery smooth muscle cells, under physiological conditions. Thus it is likely to contribute to the resting membrane potential of these cells.