Background: Cryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and provide disease- and treatment-relevant data in clinical studies. Here, we developed combined protein (40 antibodies) and transcript single-cell (sc)RNA sequencing (scRNA-seq) in PBMCs. Results: Among 31 participants in the Women’s Interagency HIV Study (WIHS), we sequenced 41,611 cells. Using Boolean gating followed by Seurat UMAPs (tool for visualizing high-dimensional data) and Louvain clustering, we identified 50 subsets among CD4+ T, CD8+ T, B, NK cells, and monocytes. This resolution was superior to flow cytometry, mass cytometry, or scRNA-seq without antibodies. Combined protein and transcript scRNA-seq allowed for the assessment of dise...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
AbstractThe use of peripheral blood mononuclear cells (PBMC) for transcriptome analysis has already ...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
BackgroundCryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and prov...
Summary: The molecular characterization of immune subsets is important for designing effective strat...
The molecular characterization of immune subsets is important for designing effective strategies to ...
High-throughput single-cell RNA Sequencing (scRNA-seq) is a powerful platform for the characterisati...
Background: Peripheral blood mononuclear cells (PBMCs) are widely used as a model in the study of di...
Summary: Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows...
IntroductionPeople living with HIV (PLHIV) are characterized by functional reprogramming of innate i...
Monitoring changes in the immune profile in blood samples can help identifying changes in tumor bio...
BackgroundSingle-cell RNA sequencing (scRNA-seq) provides high-dimensional measurements of transcrip...
Infections of the human immunodeficiency virus (HIV) trigger host immune responses, but the virus ca...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
AbstractThe use of peripheral blood mononuclear cells (PBMC) for transcriptome analysis has already ...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
BackgroundCryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and prov...
Summary: The molecular characterization of immune subsets is important for designing effective strat...
The molecular characterization of immune subsets is important for designing effective strategies to ...
High-throughput single-cell RNA Sequencing (scRNA-seq) is a powerful platform for the characterisati...
Background: Peripheral blood mononuclear cells (PBMCs) are widely used as a model in the study of di...
Summary: Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows...
IntroductionPeople living with HIV (PLHIV) are characterized by functional reprogramming of innate i...
Monitoring changes in the immune profile in blood samples can help identifying changes in tumor bio...
BackgroundSingle-cell RNA sequencing (scRNA-seq) provides high-dimensional measurements of transcrip...
Infections of the human immunodeficiency virus (HIV) trigger host immune responses, but the virus ca...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...
AbstractThe use of peripheral blood mononuclear cells (PBMC) for transcriptome analysis has already ...
BackgroundThe latent HIV reservoir represents the major barrier to a cure. One curative strategy is ...