Enzymatic hydrolysis of protein in shrimp waste

Enzimi su biološki katalizatori koji pri definiranim uvjetima ubrzavaju biokemijske reakcije u živim stanicama i izvan njih. Proteaze su enzimi koji kataliziraju hidrolizu peptidne veze u proteinima. U eksperimentalnom dijelu ovog rada korištene su tri komercijalne proteaze: Alkalaza, Novozyme 37071 i Novo-Pro D, s ciljem hidrolize proteina u otpadu ljuski rakova. Navedeni proces je jedan od koraka izolacije hitina iz ove vrste otpada. Ova biološka metoda uklanjanja proteina ima brojne prednosti ispred kemijskih metoda. Rezultati pokazuju da su sve tri proteaze uspješne u reakciji hidrolize proteina iz otpada rakova, dok je najveći učinak pokazao Novo – Pro D.Enzymes are biological catalysts that, under defined conditions, accelerate biochemical reactions inside and outside living cells. Proteases are enzymes that catalyse the cleavage of peptide bonds in proteins. Three commercial proteases were used in this work: Alcalase, Novozyme 37071 and Novo – Pro D, with the aim of hydrolysing proteins in shrimp waste. The mentioned method is one of the steps in the isolation of chitin from this type of waste. This biological method of protein removal has more advantages than chemical methods. The results show that all three proteases are successful in the protein hydrolysis reaction from shrimp waste, while Novo – Pro D showed the greatest effect