FIGURE 3 from Signaling Pathway Alterations Driven by BRCA1 and BRCA2 Germline Mutations are Sufficient to Initiate Breast Tumorigenesis by the PIK3CA^H1047R Oncogene

Comparison of breast epithelial cells of BRCA1 and BRCA2 mutation carriers with that of non-carriers. Breast epithelial cells were subclassified into BM, LASP, and LHS cells based on CD49f and EpCAM expression status as described previously (10). A, Side-by-side comparison of breast epithelial cells of BRCA1 mutation carriers with that of non-carriers. A distinct cluster of LASP cells is present only in non-carriers (indicated by an oval shape). B, Side-by-side comparison of breast epithelial cells of BRCA2 with that of non-carriers. Similar to BRCA1, a distinct cluster of LASP cells is present only in non-carriers. C, Side-by-side comparison of breast epithelial cells from BRCA1 mutation carriers with that of BRCA2 mutation carriers. D, Distribution pattern of BM, LASP, and LHS cells in non-carrier, BRCA1 and BRCA2 mutation carriers. E, Heat map showing top 10 genes highly expressed in cluster missing in BRCA1 and BRCA2 mutation carriers compared with other clusters.