Collagen-based biomaterials in organoid technology for reproductive medicine: composition, characteristics, and applications

Abstract Collagen-based biomaterials (CBB) are highly esteemed by researchers in materials science and biomedicine due to their extensive applications across various biomedical disciplines. In recent years, owing to advancements in developmental biology techniques, this superior biomaterial has seen increasing utilization in 3D in vitro tissue culture. Three-dimensional cell cultures, often referred to as organoids, have emerged in response to technological advancements in biomaterials and the growing need in the field of medical research. They serve as important models for simulating normal physiological activities in vivo, addressing limitations in experimental material sources, and resolving ethical issues. In this review, we discuss the material characteristics of CBBs commonly used for organoid culture, integrating aspects such as Matrigel and decellularized ECM as culture matrices. We also analyzed the development prospects and directions of various materials in the context of biology, clinical medicine, and particularly reproductive medicine. Currently, despite the FDA approval and clinical research incorporating numerous CBBs, existing challenges in multiple studies indicate a significant unmet need in the development of key tissue models for both medical research and clinical applications. In summary, CBBs are swiftly broadening their applicability in the realms of organoid nature and medical research, serving as a versatile and high-performing material for 3D in vitro tissue culture. Graphical abstrac