STAT6 KO-adapted N. brasiliensis elicit a stronger inflammatory WT host immune response. Mesenteric lymph node data (A-K) A. Experimental design; WT or STAT6 KO (generation 25 or 24, respectively) adapted worms infected WT mice, and flow cytometry was run on cells from mesenteric lymph nodes at day 8 post infection. B. Gating strategy. C. Total number of mesenteric lymph node cells. D-E. Percent and total cell number of IL-13+ Th2 cells. F-G. Percent and total cell number of ST2+ Th2 cells. H-I. Percent and total cell number of IL-17+ γδT cells. J-K. Percent and total cell number of IFNγ+ CD8+ T cells in mesenteric lymph nodes. Data from the peritoneal cavity (L-T). L. Gating strategy to detect myeloid cell populations; cell types outlined ...
<p>Wild-type (C57BL/6) and CD28<sup>−/−</sup> mice were infected with 500 L3 <i>N. brasiliensis</i> ...
N. brasiliensis has an altered transcriptional profile within STAT6 KO hosts. A. Experimental design...
<p>MB<sup>cre</sup>IL-4Rα<sup>−/lox</sup> and IL-4Rα<sup>−/lox</sup> mice were infected for 1 day wi...
Mesenteric lymph node data (A-J) A. Experimental design; Parental worms infected WT or STAT6 KO mice...
<p>Surface expression of CD28 and TCR on CD4<sup>+</sup> T cells and CD86 and MHCII on B cells in na...
<p>B cells were isolated from WT or MyD88<sup>−/−</sup> mice 24 hours post <i>N. brasiliensis</i> in...
Supernatant from 3-day cultures of WT or STAT6 KO- adapted (G24/23) N. brasiliensis was used to stim...
STAT6 KO-adapted N. brasiliensis do not exhibit attenuation of fitness when challenged within WT mic...
<p><i>N. brasiliensis</i> infected μMT and BALB/c mice were re-infected with 500xL3 larvae and at da...
<p><i>MB</i>1<sup>Cre</sup>IL-4Rα<sup>−/lox</sup> and IL-4Rα<sup>−/lox</sup> mice were infected for ...
<p>iLck<sup>cre</sup>IL-4Rα<sup>−/lox</sup> and control mice were infected with 750 <i>N. brasiliens...
A. Experimental evolution design, N. brasiliensis (originally isolated from rat host, brown) were in...
<p>Mice were infected with 500 L3 <i>N</i>. <i>brasiliensis</i>, fed with tamoxifen once daily from ...
<p>IL-4Rα<sup>−/−</sup> and IL-4Rα<sup>−/lox</sup> mice were infected for 5 or 7 days post-secondary...
Summary of regression analysis of host immune cell populations versus hookworm adaptation genotypes,...
<p>Wild-type (C57BL/6) and CD28<sup>−/−</sup> mice were infected with 500 L3 <i>N. brasiliensis</i> ...
N. brasiliensis has an altered transcriptional profile within STAT6 KO hosts. A. Experimental design...
<p>MB<sup>cre</sup>IL-4Rα<sup>−/lox</sup> and IL-4Rα<sup>−/lox</sup> mice were infected for 1 day wi...
Mesenteric lymph node data (A-J) A. Experimental design; Parental worms infected WT or STAT6 KO mice...
<p>Surface expression of CD28 and TCR on CD4<sup>+</sup> T cells and CD86 and MHCII on B cells in na...
<p>B cells were isolated from WT or MyD88<sup>−/−</sup> mice 24 hours post <i>N. brasiliensis</i> in...
Supernatant from 3-day cultures of WT or STAT6 KO- adapted (G24/23) N. brasiliensis was used to stim...
STAT6 KO-adapted N. brasiliensis do not exhibit attenuation of fitness when challenged within WT mic...
<p><i>N. brasiliensis</i> infected μMT and BALB/c mice were re-infected with 500xL3 larvae and at da...
<p><i>MB</i>1<sup>Cre</sup>IL-4Rα<sup>−/lox</sup> and IL-4Rα<sup>−/lox</sup> mice were infected for ...
<p>iLck<sup>cre</sup>IL-4Rα<sup>−/lox</sup> and control mice were infected with 750 <i>N. brasiliens...
A. Experimental evolution design, N. brasiliensis (originally isolated from rat host, brown) were in...
<p>Mice were infected with 500 L3 <i>N</i>. <i>brasiliensis</i>, fed with tamoxifen once daily from ...
<p>IL-4Rα<sup>−/−</sup> and IL-4Rα<sup>−/lox</sup> mice were infected for 5 or 7 days post-secondary...
Summary of regression analysis of host immune cell populations versus hookworm adaptation genotypes,...
<p>Wild-type (C57BL/6) and CD28<sup>−/−</sup> mice were infected with 500 L3 <i>N. brasiliensis</i> ...
N. brasiliensis has an altered transcriptional profile within STAT6 KO hosts. A. Experimental design...
<p>MB<sup>cre</sup>IL-4Rα<sup>−/lox</sup> and IL-4Rα<sup>−/lox</sup> mice were infected for 1 day wi...