<i>Gr64af-Gal4</i>-positive neurons project in similar patterns in the CNS of <i>D</i>. <i>suzukii</i> and <i>D</i>. <i>melanogaster</i> and are not required for oviposition responses to acetic acid.

(A) Axonal projection patterns of the neurons labeled by the Gr64af-Gal4 lines from D. melanogaster (top row) and D. suzukii (bottom row) in the CNS. A UAS-Cd4tdTomato reporter was used in both species, neuropil stained with nc82 antibody (magenta). Gr64af-Gal4-positive neurons project exclusively to the gustatory center—the sub-eosophageal zone (SEZ)—in both species. Merged images (far left) show z-projections over all planes. Images on the right and in the green channel-only show z-projections at different depths from anterior to posterior, revealing similar categories of arborization patterns in both species. (B) Two-choice oviposition assays opposing acetic acid at the indicated concentrations to plain agar, in the presence of 1% casein hydrolysate on both sides to stimulate egg-laying. Sugar-GRN inhibition does not abolish the response of either species to acetic acid (n = 28, 29, 30, 30, 30, 30, 30, 30, 30, 25, 22, 18, 25, 22, 19, 27, 23, 18). The data underlying this figure can be found in S3 Data. (EPS)