The pressure to plant seedlings in unprepared instead of prepared soil in forest regeneration is increasing, although seedlings’ growth in unprepared soil is poorer. One way to improve seedlings’ growth could be to add arginine phosphate to the planting hole at the time of planting. In field studies, abiotic and biotic damage normally disturbs studies which seek to determine growth effects, especially in unprepared soil. The study’s aims were (i) to clarify whether it was possible to investigate the differences between varying soil layer structures mimicking site preparation methods and seedling treatments in simulated planting places in partly controlled conditions; and (ii) to investigate the effects of arginine phosphate (arGrow® Granula...